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Summary

This module will provide an introduction to fundamental contemporary molecular laboratory techniques used in cardiovascular research. Students will undertake practical work covering fundamental laboratory skills and more advanced genetic/molecular biology methods such as genetic screening, sequencing methods and molecular cloning. Topics related to practical sessions will also be covered by lectures, exercises, extensive e-learning, workshops and tutorials.

The module is founded on research into the genetic basis of inherited cardiomyopathies and cross-links to taught sessions in the Cardiovascular Science & Disease (CARD0004) and Genetics of Cardiovascular and Related Complex Diseases (GENE0002) modules.

Learning Outcomes and Objectives

Students successfully completing this module should:

• Have practised fundamental laboratory methods in a research laboratory, or if laboratory access is restricted, via online methods
• Describe and debate the key laboratory techniques taught in this module, showing good understanding of the theoretical concepts
• Have experienced and be able to use bioinformatic resources tools related to molecular cardiovascular research in the analysis of genetic data

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Who is this module for?

This is an optional module for MSc Cardiovascular Science students and is open to any ÐÂÏã¸ÛÁùºÏ²Ê¿ª½±½á¹ûMSc/PG Dip student from an allied subject within SLMS.

Teaching and Learning Methods

Learning will be through practical sessions, lectures, computer exercises, e-learning (online resources, videos and simulations of molecular laboratory methods) in addition to tutorials.

Selected Reading List

Human molecular genetics 4. Tom Strachan and Andrew Read. New York; Abingdon: Garland Science 4th ed. 2011

Edwards J, Bartlett JM. Mutation and polymorphism detection: a technical overview. Methods Mol Biol. 2003;226:287-94.

George AL Jr. Use of contemporary genetics in cardiovascular diagnosis. Circulation. 2014;130(22):1971-80.

Pearson H, Stirling D. Cloning and mutagenesis: a technical overview. Methods Mol Biol 2003;226:464-499.